[Retracted] circZC3HAV1 Regulates TBC1D9 to Affect the Biological Behavior of Colorectal Cancer Cells

Figure 3

circZC3HAV1 competitively binds to miR-146b-3p in CRC cells. (a,b) The localization of circZC3HAV1 in CRC cells was detected through subcellular fractionation and FISH assays. Scale bar: 10 μm. (c) RIP assay was conducted to detect the enrichment of circZC3HAV1 in anti-AGO2. (d) circBank database was used to project the potential miRNAs which might bind to circZC3HAV1. (e) The binding affinities between circZC3HAV1 and candidate miRNAs were evaluated by RNA pull down assay. (f) RIP assay was performed to detect the enrichment of circZC3HAV1 and miR-146b-3p in anti-AGO2 by RIP assay. (g) The binding of circZC3HAV1 and miR-146b-3p was tested by RNA pull down assay. (h) In 293T cells, luciferase reporter assay was implemented to examine the luciferase activity of circZC3HAV1 when miR-146b-3p was upregulated. (i) The miR-146b-3p expression was examined by qPCR before and after the overexpression of circZC3HAV1 in CRC cells. .

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