[Retracted] circZC3HAV1 Regulates TBC1D9 to Affect the Biological Behavior of Colorectal Cancer Cells

Figure 4

circZC3HAV1 regulates CRC cell migration and invasion by sponging miR-146b-3p. (a) The overexpression efficacy of miR-146b-3p mimics was measured by qPCR in CRC cells. (b) Wound healing assay was utilized for detecting the migratory capability of CRC cells in response to miR-146b-3p mimics. Scale bar: 100 μm. (c, d) The migratory and invasive abilities of CRC cells were detected by Ttranswell assays after overexpressing miR-146b-3p. Scale bar: 100 μm. (e) After the overexpression of miR-146b-3p, qPCR was utilized to detect the expression of MMP2 and MMP9. (f) Wound healing assay was performed to detect CRC cell migration under different conditions. (g) Migratory and invasive processes of CRC cells were evaluated by Transwell assays upon indicated conditions. (h) The expression of MMP2 and MMP9 in CRC cells transfected with different plasmids was detected by qPCR. .

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