[Retracted] circZC3HAV1 Regulates TBC1D9 to Affect the Biological Behavior of Colorectal Cancer Cells

Figure 5

circZC3HAV1 regulates TBC1D9 expression via competitively adsorbing miR-146b-3p. (a) DIANA tools database was used to detect candidate mRNA that might bind to miR-146b-3p. (b, d) The interference efficiency of target mRNAs was measured by qPCR in CRC cells. (e) After LMTK2 downregulation, the migration of CRC cells was monitored in wound healing assays. Scale bar: 100 μm. (f) After LMTK2 knockdown, MMP2 and MMP9 expressions in CRC cells were detected by qPCR. (g) After TBC1D9 deficiency, the migration of CRC cells was tested by wound healing assays. Scale bar: 100 μm. (h) Upon TBC1D9 depletion, MMP2 and MMP9 expressions in CRC cells were detected by qPCR. (i) After downregulation of TP53INP2, the migration of CRC cells was examined via wound healing assays. Scale bar: 100 μm. (j) After knockdown of TP53INP2, the expression of MMP2 and MMP9 in CRC cells was detected by qPCR. (k) The enrichment of circZC3HAV1, miR-146b-3p, and TBC1D9 were detected by RIP assay in CRC cells. (l) Luciferase reporter assay was carried out to detect the influence of circZC3HAV1 overexpression on the binding of TBC1D9 and miR-146b-3p in 293T cells. (m) CRC cell migration was detected by wound healing assay under different conditions. (n) Transwell assay was applied for assessing migration and invasion of CRC cells transfected with different plasmids. (o) MMP2 and MMP9 expressions were detected by qPCR in CW-2 cells transfected with indicated plasmids. .

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