[Retracted] Histone Deacetylase9 Represents the Epigenetic Promotion of M1 Macrophage Polarization and Inflammatory Response via TLR4 Regulation

Figure 2

RAW264.7 macrophage expression levels of HDAC9, CD38, IL-1β, and TNF-α, and immunofluorescence CD38 in the control group, ox-LDL group, ox-LDL + HDAC9-shRNA group, and ox-LDL + NC-shRNA transfection group. (a) HDAC9, CD38, IL-1β, and TNF-α protein expression WB bands in the above four groups. (b) Histogram of HDAC9 protein expression in the above four groups. (c) Histogram of CD38 protein expression in the above four groups. (d) Histogram of IL-1β protein expression in the above four groups. (e) Histogram of TNF-α protein expression in the above four groups. (f) Immunofluorescence images of CD38 protein expression in the above four groups. Blue: nucleus; red: CD38 protein; blend: blue and red overlay. (control group versus ox-LDL group), (ox-LDL group versus ox-LDL + HDAC9-shRNA group), (ox-LDL + HDAC9-shRNA group versus ox-LDL + NC-shRNA group), ^ns (ox-LDL group versus ox-LDL + NC-shRNA group).

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