hsa-miR-206b Involves in the Development of Papillary Thyroid Carcinoma via Targeting LMX1B

Figure 5

LMX1B was a target gene of hsa-miR-206. (a) The LMX1B expression level was decreased and the hsa-miR-206 expression level was increased in the stage III_IV group () compared with the stage I_II group () based on TCGA data. Boxes represented the interquartile range of the data, and the lines across the boxes indicated the median values. The hash marks above and below the boxes indicated the 90^th and 10^th percentiles for each group, respectively. values were calculated by the Wilcoxon rank sum test. (b) The LMX1B expression level and hsa-miR-206 expression level were analyzed in the stage I and stage II patients based on qPCR data. Boxes represented the interquartile range of the data, and the lines across the boxes indicated the median values. The hash marks above and below the boxes indicated the 90^th and 10^th percentiles for each group, respectively. values were calculated by the Wilcoxon rank sum test. (c) A significant inverse correlation was observed between the LMX1B and hsa-miR-206 expression levels in the PTC tissue samples () by Pearson’s correlation () based on qPCR data. (d) Based on line regression, a negative correlation between the LMX1B expression levels and MACIS scores and a positive correlation between the hsa-miR-206 expression levels and MACIS scores were observed. (e) Predicted interaction between hsa-miR-206 and the putative binding sites in the LMX1B 3-UTR based on miRanda. hsa-miR-206 seed sequence was shown in bold. The representation was limited to the region around the hsa-miR-206 complementary site. The number of the binding site was 1959-1981.

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