ERK1/2-Dependent Inhibition of Glycolysis in Curcumin-Induced Cytotoxicity of Prostate Carcinoma Cells

Figure 1

Effects of curcumin on cell viability and expression of key enzymes in glucose metabolism. PC-3AcT and DU145AcT cells were treated with the indicated concentrations of curcumin in DMEM containing 3.8 μM lactic acid for 48 h. Human prostate epithelial cell lines HPrEC were incubated in lactic acid-free medium. (a) Cell viability (% control). (b) The expression levels of p-MEK and p-ERK1/2. (c) The expression levels of rate-limiting enzymes in glucose metabolism. (d) Activities of hexokinase and pyruvate dehydrogenase (% control). (e) The expression levels of complexes I-V in the mitochondrial electron transport chain. ^# vs. respective HPrEC cells. vs. respective control cells. CCM: curcumin; HK: hexokinae; PFKP: phosphofructokinase platelet; PDH: pyruvate dehydrogenase. NDUFB8: NADH:ubiquinone oxidoreductase subunit B8 (complex I); SDHB: succinate dehydrogenase complex iron sulfur subunit B (complex II); UQCRC2: ubiquinone-cytochrome C reductase core protein 2 (complex III); COX II: mitochondrial cytochrome C oxidase subunit II (complex IV); ATP5A: ATP synthase F1 subunit alpha (complex V).

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