ERK1/2-Dependent Inhibition of Glycolysis in Curcumin-Induced Cytotoxicity of Prostate Carcinoma Cells

Figure 3

Effects of curcumin and/or PD98059 treatment on cell viability and aerobic glycolysis. PC-3AcT and DU145AcT cells were treated with curcumin (40 μM) and PD98059 (50 μM), alone or in combination, in DMEM containing 3.8 μM lactic acid for 48 h. Human prostate epithelial cell lines HPrEC were incubated in lactic acid-free medium. (a) Cell viability. The percentage of viable cells was determined by comparison to the results obtained using DMSO-treated control cells (100%). (b) The expression levels of p-MEK and p-ERK1/2. (c) The expression levels of rate-limiting enzymes in glucose metabolism. (d) Activities of hexokinase and pyruvate dehydrogenase (% control). (e) Glucose concentration (% control) in culture medium. vs. respective control cells. Different letters above error bars are significantly different (). CCM: curcumin; PD: PD98059; HK: hexokinae; PFKP: phosphofructokinase platelet; PDH: pyruvate dehydrogenase.

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