Validated HPLC-UV Method for Simultaneous Estimation of Paclitaxel and Doxorubicin Employing Ion Pair Chromatography: Application in Formulation Development and Pharmacokinetic Studies

Figure 4

Application of the developed method in evaluation of the combination formulation. (a) Multilamellar liposomal formulation coloaded with the PTX and DOX in lipid and aqueous compartment, respectively. (b) Chromatogram depicting specificity of the analytes with respect to the formulation components. (c) Matrix effect of the formulation components on analysis of PTX and DOX, (d) entrapment efficiency, and (e) loading efficiency of the developed formulation analyzed by the developed HPLC method.

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