Validated HPLC-UV Method for Simultaneous Estimation of Paclitaxel and Doxorubicin Employing Ion Pair Chromatography: Application in Formulation Development and Pharmacokinetic Studies

Table 3

Stability of PTX and DOX samples expressed as percentage of their initial concentration on storage for 3 days under refrigerated and room temperature condition.

Analyte	Concentration	Time (hrs)	Recovery (%)	% RSD
Intraday (5°C)
PTX	1.0	0	101.06	0.04
		12	101.08	0.07
DOX	1.0	0	100.21	0.76
		12	99.46	1.30
Interday (5°C)
PTX	1.0	24	100.21	0.55
		48	100.09	0.64
		72	99.03	1.38
DOX	1.0	24	98.83	1.75
		48	98.45	2.02
		72	96.06	3.71
Intraday (25°C)
PTX	1.0	0	101.06	0.04
		12	101.41	0.30
DOX	1.0	0	100.21	0.76
		12	99.15	1.50
Interday (25°C)
PTX	1.0	24	99.52	1.04
		48	99.03	1.38
		72	98.12	2.02
DOX	1.0	24	96.97	3.05
		48	96.14	3.64
		72	96.06	3.71