Validated HPLC-UV Method for Simultaneous Estimation of Paclitaxel and Doxorubicin Employing Ion Pair Chromatography: Application in Formulation Development and Pharmacokinetic Studies

Table 4

Robustness expressed in percent mean recovery and percent relative standard deviation for PTX and DOX.

Parameter	Rt	Recovery (%)	%RSD
PTX
Mobile-phase ACN : water (68 : 32)	3.67	99.82	0.138
Mobile-phase ACN : water (63 : 37)	4.41	98.20	1.318
Mobile-phase ACN : water (58 : 42)	5.60	98.77	0.906
Flow rate (0.9 ml/min)	4.86	102.46	1.804
Flow rate (1.0 ml/min)	4.38	97.77	1.638
Flow rate (1.1 ml/min)	3.99	99.44	0.413
Column temp. (40°C)	4.36	98.30	1.256
Column temp. (35°C)	4.39	97.50	1.818
Column temp. (30°C)	4.40	99.03	0.679
DOX
Mobile phase (68 : 32)	7.53	100.64	0.451
Mobile phase (63 : 37)	7.46	101.05	0.744
Mobile phase (58 : 42)	7.28	99.59	0.297
Flow rate (0.9 ml/min)	7.74	100.34	0.240
Flow rate (1.0 ml/min)	7.48	100.68	0.485
Flow rate (1.1 ml/min)	6.35	99.07	0.660
Column temp. (40°C)	6.95	100.98	0.698
Column temp. (35°C)	7.34	100.85	0.603
Column temp. (30°C)	7.39	101.64	1.154