Polyacetylenes from Codonopsis lanceolata Root Induced Apoptosis of Human Lung Adenocarcinoma Cells and Improved Lung Dysbiosis

Figure 1

Effects of CLP on the proliferation of A549 cells. (a) HPLC chromatograph of standards (above) and CLP (below). The peaks marked with 1~6 were syringin, codonopilodiynoside A, lobetyol, (+)-isolariciresinol, lobetyolin, and atractylenolide III, respectively. (b) Effects of CLP on the viability of A549 cells. The A549 cells were treated with 1.25~20 μg/mL of CLP for 24, 48, and 72 h. (c) Effects of CLP on the viability of normal lung epithelial BEAS-2B cells. Cell viability was determined by MTT assay. The BEAS-2B cells were treated with 20 μg/mL of CLP for 24, 48, and 72 h, respectively. (d) Effects of compounds isolated from CLP on viability of A549 cells. The A549 cells were treated with 20 μg/mL of different compounds for 48 h. (e) The contributions of the main bioactivity compounds to the inhibition rates of CLP on the proliferation of A549 cells. The A549 cells were treated with 9.92 μg/mL of syringin, 1.82 μg/mL of lobetyol, and 2.06 μg/mL of lobetyolin for 48 h, which represented their concentrations in 20 μg/mL CLP. (f) The synergistic inhibition of lobetyol and lobetyolin on the proliferation of A549 cells, which was calculated by using CompuSyn software. Data were expressed as . The groups marked with different letters suggested significant differences, .

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