IL-17A Promotes the Migration, Invasion and the EMT Process of Lung Cancer Accompanied by NLRP3 Activation

Figure 3

IL-17A facilitated the migration and invasion in lung cancer cells. (a) Wound healing assay showed that IL-17A administration accelerate wound healing of A549 cells. (b) Wound healing rate was calculated as (wound width of time zero–that of each time point) ÷ wound width of time zero ×100%. (c) Transwell invasion assay showed that IL-17A promotes invasion of A549 cells. Magnification, ×100. (d) Number of the cells invaded through the membrane. (e) Representative real-time traces of cell index showed that IL-17A administration promotes A549 migration. (f) Wound healing assay showed that IL-17A administration accelerate wound healing of H1299 cells. (g) Wound healing rate was calculated as (wound width of time zero–that of each time point) ÷ wound width of time zero ×100%. (h) Transwell invasion assay showed that IL-17A promotes invasion of H1299 cells. (i) Number of the cells invaded through the membrane. (j) Representative real-time traces of cell index showed that IL-17A administration promotes A549 migration. One-way ANOVA. Error bars, mean ± SD. P <0.05; P <0.01; P <0.001, ns, non-signifcant; SD, standard deviation.