IL-17A Promotes the Migration, Invasion and the EMT Process of Lung Cancer Accompanied by NLRP3 Activation

Figure 6

NLRP3 siRNA inhibited IL-17A induced migration and invasion. (a) Wound-healing assay showing that NLRP3 knockdown prevent migration induced by IL-17A in A549 cells. (b) Wound healing rate was calculated as (wound width of time zero–that of each time point) ÷ wound width of time zero ×100%; (c) NLRP3 siRNA impaired invasion A549 cells through Matrigel. Magnification, ×100. (d) Number of the cells invaded through the membrane. (e) Representative real-time traces of CI showed that siNLRP3 inhibit IL-17A induced migration in A549 cells. (f) Wound-healing assay showing that NLRP3 knockdown prevent migration induced by IL-17A in H1299 cells. (g) Wound healing rate in each group of H1299 cells. (h) NLRP3 siRNA impaired invasion H1299 cells through Matrigel. Magnification, ×100. (i) Number of the cells of H1299 invaded through the membrane in each group. (j) Representative real-time traces of CI showed that siNLRP3 inhibit IL-17A induced migration in H1299 cells. Student’s t-test, mean ± SD are given, P <0.05; P <0.01; P <0.001, ns, non-signifcant. CI, cell index.