IL-17A Promotes the Migration, Invasion and the EMT Process of Lung Cancer Accompanied by NLRP3 Activation

Figure 7

NLRP3 knockdown prevented IL-17A induced the EMT process. (a) Western blot of several EMT related markers indicated that NLRP3 siRNA increase E-cadherin level while decrease vimentin and α-SMA level induced by IL-17A administration in A549 cells. (b) Quantification of results from A. (c) Immunofluorescence staning of vimentin in A549 cells in each group. Magnification, ×400. (d) Immunofluorescence staning of E-cadherin in A549 cells in each group. Magnification, ×400. (e) The expression of EMT and NLRP3-related markers in each group of H1299. (f) Quantification of results from e. (g) Immunofluorescence staning of vimentin in H1299 cells in each group. Magnification, ×400. (h) Immunofluorescence staning of E-cadherin in H1299 cells in each group. Magnification, ×400. Student’s t-test, mean ± SD are given, P <0.05; P <0.01; P <0.001, ns, non-signifcant. EMT, epithelial-mesenchymal transition.

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