SLC6A14 Depletion Contributes to Amino Acid Starvation to Suppress EMT-Induced Metastasis in Gastric Cancer by Perturbing the PI3K/AKT/mTORC1 Pathway

Figure 1

SLC6A14 expression is positively induced in GC cells with strong metastatic ability. mRNA expression profiles in MKN28-M compared with MKN-28-NM (a–c) and in SGC7901-M compared with SGC7901-NM cells (d–f) were confirmed using mRNA microarray analysis. (a, d) Heat map depicting differentially expressed genes (DEGs), in which the upregulated and downregulated genes were represented by red and green columns, respectively (). (b, e) The scatter plot indicating the distribution of signal intensity between different treatment groups on the rectangular coordinate plane. (c, f) Volcanic maps displaying significant difference were drawn based on the fold change and values. (g) The Venn diagram showing 10 intersectional upregulated genes with a fold in their mRNA expressions in both MKN28-M and SGC7901-M, compared with MKN28-NM and SGC7901-NM cells, respectively. (h) Representative images of High-Content shRNA Screening for GATA6, PGM2L1, ARL2, SAMD13, CST1, ADAMTSL4, SPINK4, ACY3, RGS2, and SLC6A14 were obtained from wound healing assay (upper panels). The 2-day migration rate of MKN28-M cells that were transfected with indicated shRNAs was also demonstrated as the of three replicates (bottom panel). versus control. (i) Fold change of migration rate in MKN28-M cells with indicated transfections was calculated and was shown as the from three independent experiments. versus control. (j) SLC6A14 expression in different GC cell lines was checked using Western blot analysis. Data were represented as the strip maps (left panels) and relative gray values towards β-actin with the from three irrelevant experiments (right panel). versus the SGC7901-NM and MKN28-NM group. (k) The migration capability of indicated GC cells was evaluated by wound healing assay. Data were demonstrated as the morphology of “wound strack” (left panels) and were also judged as the migration rate with the from three triplicate experiments (right panel). versus the SGC7901-NM and MKN28-NM group.

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