SLC6A14 Depletion Contributes to Amino Acid Starvation to Suppress EMT-Induced Metastasis in Gastric Cancer by Perturbing the PI3K/AKT/mTORC1 Pathway

Figure 2

SLC6A14 upregulation is a risk stratification factor for GC patients with metastasis and poor prognosis. (a) Quantitative study of SLC6A14 mRNA in GC and paired ANTs was carried out by Quantitative Real-time PCR analysis. Data are demonstrated as the relative expression towards GAPDH with the from three independent experiments. . (b) Protein level of SLC6A14 in GC and corresponding ANTs was determined using Western blot assay. Data are shown as the representatives (upper panels) and the relative expression towards β-actin with the of three replicates (bottom panels). . (c) SLC6A14 expression in serial samples from GC patients was examined by immunohistochemical staining. Data are manifested as the representative images (upper panels) and staining scores for SLC6A14 with the from three independent experiments (bottom panel). . (d) Overall survival (OS) and (e) disease-free survival (DFS) curves of GC patients were generated using Kaplan-Meier analysis, and the correlation between SLC6A14 expression and OS or DFS time was evaluated by log-rank test. were considered to be statistically significant.

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