Melatonin Ameliorates the Progression of Alzheimer’s Disease by Inducing TFEB Nuclear Translocation, Promoting Mitophagy, and Regulating NLRP3 Inflammasome Activity

Figure 2

(a) The levels of mitophagy-related proteins PINK1, Parkin, and p62 and the inflammasome-related protein caspase-1 and NLRP3 were detected by western blotting between placebo control and MT intervention groups. (b) Immunofluorescence method was used to detect the mitophagy-related proteins PINK1 and LC3. (c) The oxidative activated oxygen (ROS) of animal tissue was detected by luminol chemiluminescence kit between placebo control and MT intervention groups. (d) Expression of IL-1β, IL-6, and IL-18 mRNA was detected by qRT-PCR between placebo control and MT intervention groups. Data are presented as . ; ; .

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