Melatonin Ameliorates the Progression of Alzheimer’s Disease by Inducing TFEB Nuclear Translocation, Promoting Mitophagy, and Regulating NLRP3 Inflammasome Activity

<div>The AD cell model was constructed in SH-SY5Y cells with A<i>β</i><sub>25-35</sub> treatment. (a) The levels of mitophagy-related proteins Parkin and p62 and the inflammasome-related protein caspase-1 were detected by western blotting with the MT treatment. (b) The colocalization between LC3 and PINK1 was detected by immunofluorescence with the MT treatment. (c) The lever of ROS was detected by luminol chemiluminescence kit with the MT treatment. (d) Expression of IL-1<i>β</i> and IL-18 mRNA was detected by qRT-PCR with the MT treatment. (e) Combined with MT and hydroxychloroquine (HCQ), the lever of ROS was detected by the luminol chemiluminescence kit. (f) Combined with MT and HCQ, the expression of IL-1<i>β</i> and IL-18 mRNA was detected by qRT-PCR. 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BioMed Research International

fig3

Figure 3

Figure 3: Melatonin Ameliorates the Progression of Alzheimer’s Disease by Inducing TFEB Nuclear Translocation, Promoting Mitophagy, and Regulating NLRP3 Inflammasome Activity 

Figure 3 | Melatonin Ameliorates the Progression of Alzheimer’s Disease by Inducing TFEB Nuclear Translocation, Promoting Mitophagy, and Regulating NLRP3 Inflammasome Activity 