Melatonin Ameliorates the Progression of Alzheimer’s Disease by Inducing TFEB Nuclear Translocation, Promoting Mitophagy, and Regulating NLRP3 Inflammasome Activity

Figure 4

(a) Western blotting analysis of the expression of TFEB in nucleus was performed in APP/PS1 transgenic mice. (b) SH-SY5Y cells overexpressing TFEB were treated with a combination of Aβ25-35, and the expressions of TFEB, Parkin, p62, and caspase-1 were detected by western blotting. (c) SH-SY5Y cells overexpressing TFEB were treated with a combination of Aβ25-35, and the colocalization between LC3 and PINK1 was detected by immunofluorescence. (d) SH-SY5Y cells overexpressing TFEB were treated with a combination of Aβ25-35, and the oxidative activated oxygen (ROS) was detected by luminol chemiluminescence kit. (e) SH-SY5Y cells overexpressing TFEB were treated with a combination of Aβ25-35, and the expression of IL-1β and IL-18 was analysed. Data are presented as . ; ; .

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