Genetic Diversity of Escherichia coli Coharboring mcr-1 and Extended Spectrum Beta-Lactamases from Poultry

Table 1

PCR primers list for amplifications of resistance genes determinates.

Target gene name	Primers (name)	Sequences	Annealing (temperature)	Product
uidA	(uid-A)-(F); (uid-A)-(R)	CAACGAACTGAACTGGCAGA; CATTACGCTGCGATGGAT	58	162
blaCTX-M	CTX-MU-(F); CTX-MU-(R)	ATGTGCAGYACCAGTAARGT; TGGGTRAARTARGTSACCAGA	52	593
blaCTX-M-1	CTX-M-1-(F); CTX-M-1-(R)	GGTTAAAAAATCACTGCGTC; TTACAAACCGTYGGTGACGA	50	873
blaCTX-M-2	CTX-M-2-(F); CTX-M-2-(R)	ATGATGACTCACAGCATTCG; TCCCGACGGCTTTCCGCGTT	56	833
blaCTX-M-8	CTX-M-8-(F); CTX-M-8-(R)	TTTGCCCGTGCGATTGG; CGACTTTCTGCCTTCTGCTCT	50	368
blaCTX-M-9	CTX-M-9-(F); CTX-M-9-(R)	ATGGTGACAAAGAGAGTGCA; CCCTTCGGCGATGATTCTC	50	870
blaCTX-M-10	CTX-M-10-(F); CTX-M-10-(R)	GCAGCACCAGTAAAGTGATGG; GCGATATCGTTGGTGGTACC	56	524
blaCTX-M-14	CTX-M-14-(F); CTX-M-14-(R)	GAGAGTGCAACGGATGATG; TGCGGCTGGGTAAAATAG	56	941
blaCTX-M-15	CTX-M-15-(F); CTX-M-15-(R)	CACACGTGGAATTTAGGGACT; GCCGTCTAAGGCGATAAACA	50	995
blaTEM	TEM-(F); TEM-(R)	TTGGGTGCACGAGTGGGTTA; TAATTGTTGCCGGGAAGCTA	55	506
blaSHV	SHV-(F); SHV-(R)	ATGCGTTATATTCGCCTGTG; AGATAAATCACCACAATGCGC	56	896
mcr-1	mcr-1-(F); mcr-1-(R)	AGTCCGTTTGTTCTTGTGGC; AGATCCTTGGTCTCGGCTTG	60	320
mcr-2	mcr-2-(F); mcr-2-(R)	AGCCGAGTCTAAGGACTTGATGAATTTG; GCGGTATCGACATCATAGTCATCTTG	57	576
mcr-3	mcr-3-(F); mcr-3-(R)	CGCTTATGTTCTTTTTGGCACTGTATT; TGAGCAATTTCACTATCGAGGTCTTG-3	57	1067