[Retracted] Neuroglobin Is Involved in the Hypoxic Stress Response in the Brain

Figure 2

Expression of neuroglobin protein in neurons during the development of cerebral cortical. (a) Ngb (red) and NeuN (green) immunofluorescence double labeling staining indicated that the majority of Ngb-positive neurons presented in the cortex plate and subcortical plate and less expressed in the shallow cortical, IZ, and other parts of the cerebral cortex at E16. (b) Costaining of Ngb (red) with NeuN (green) demonstrating Ngb-immunoreactive neurons were detectable in all cortical layers, especially highly expressed in laminae V and VI at P1. (c) Ngb-positive neurons were observed in all layers of the neocortex, but Ngb-positive neurons showed lower expression in layer I at P3. (d) At P14, the laminated structure of the neocortex had fully formed. The cell body of Ngb-positive neurons at II to IV layers was smaller and arranged closely, while Ngb-positive neurons were larger at V to VI layers. (e) The lamination of the neocortex has entered its mature stage at 3 months, and Ngb-positive neurons distributed in all six layers. (f) At 1 year, the laminated structure of the neocortex has stabilized, and the expression of Ngb labeled neurons has not changed. (g–i) The stratification of the neocortex had basically formed at P7, and the boundaries among each stratum were easy to be identified. Strong Ngb-immunoreactive neurons were detected close to the molecular layer in layer II-IV, while neurons near the deep cortical layer showed moderate Ngb labelling. In laminar V to VI, Ngb-positive neurons strongly expressed near the shallow cortical, and weakly expressed in the VZ. (j) Costaining of Ngb (red) with Nestin (green) demonstrating similar distribution and expression of Ngb-positive neurons at P7 compared with (g–i). (k–m) The Ngb protein was generally expressed in the cytoplasm of the neurons under the oil microscope at P3. in (a–j); in (k–m).

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