VEGFA-Enriched Exosomes from Tendon-Derived Stem Cells Facilitate Tenocyte Differentiation, Migration, and Transition to a Fibroblastic Phenotype

Figure 1

Morphology and phenotypic identification of TDSCs and TDSC-derived exosomes. (a) Schematic presentation of the experimental design. (b) Flow cytometric analysis of cell surface markers of TDSCs. Red curves represent controls, and green curves represent surface markers. (c) TEM showing the morphology and size distribution of exosomes derived from TDSCs. Scale bar: 100 nm. (d) Western blot analyses of CD9, CD63, CD81, and TSD101 in exosomes isolated from cultured TDSCs. (e) Quantification of the expression levels of the proteins shown in (D). P <0.05 relative to TDSCs.

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