Cleavage of AUF1 by Coxsackievirus B Affects DDX5 Regulatory on Viral Replication through iTRAQ Proteomics Analysis

Figure 6

Correlations and validation of AUF1 regulation of DDX5 w/w.o viral infection. (a) Heatmap of correlations between proteins in cluster 1 and analysis of bidirectional hierarchical clustering. (b) The RT–qPCR assay was used to examine the DDX5 mRNA expression level with or without AUF1 overexpression (t test, ). GAPDH was used as an internal control. (c) DDX5 protein expression levels were detected with or without silencing AUF1 with siRNA by Western blot analysis. β-Tubulin was used as a loading control. (d) DDX5 protein levels were detected under the condition that AUF1 was cleaved during CVB infection at different MOIs and infection hours. VP1 was used for viral detection, and β-tubulin was used as a loading control. (e) CVB VP1 was examined in HeLa cells with or without EGFP-DDX5 overexpression by Western blot analysis. (f) CVB genome RNA expression levels in HeLa cells with or without EGFP-DDX5 overexpression were detected by RT-qPCR.

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