Chalcone 9X Contributed to Repressing Glioma Cell Growth and Migration and Inducing Cell Apoptosis by Reducing FOXM1 Expression In Vitro and Repressing Tumor Growth In Vivo

Figure 5

FOXM1 overexpression removed the Chalcone 9X effects on cell proliferation, apoptosis, and migration in U87 and T98G cells. P-NC and p-FOXM1 were, respectively, glioma cells, and then 100 μmol/L Chalcone 9X was treated in these cells for 48 h. (a) The mRNA levels of FOXM1 were detected by RT-PCR. (b) CCK8 assay was used to measure cell viability. (c) FACS assay was used to measure cell apoptosis. (d, e) Transwell assay was used to detect cell migration capacities. (f–h) The protein levels of Vimentin, N-cadherin, and E-cadherin in glioma cells were detected by WB (magnifications: ×0.8). .

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