Chalcone 9X Contributed to Repressing Glioma Cell Growth and Migration and Inducing Cell Apoptosis by Reducing FOXM1 Expression In Vitro and Repressing Tumor Growth In Vivo

Figure 6

Chalcone 9X inhibited U87 tumor growth of a xenograft model in nude mice. U87 cells were injected into nude mice for two weeks; then Chalcone 9X (20 mg/kg, 40 mg/kg) was provided by gavage to these mice for another four weeks. (a) Images of 9 primary tumors at day 28 from the model group, Chalcone 9X 20 mg/kg group, and Chalcone 9X 40 mg/kg group after mouse scarification. (b) Tumor volumes were measured every seven days. (c) Tumor weights on day 28 after injection was calculated. (d, e) Immunohistochemical staining of FOXM1 and Caspase-3 in tumor tissue sections. (f, g) WB assay was performed to detect the protein expressions of FOXM1, Vimentin, N-cadherin, and E-cadherin. , model group versus Chalcone 9X 20 mg/kg; , model group versus Chalcone 9X 40 mg/kg.

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