Protective Effects of the Wenfei Buqi Tongluo Formula on the Inflammation in Idiopathic Pulmonary Fibrosis through Inhibiting the TLR4/MyD88/NF-κB Pathway

Figure 3

The WBT formula alleviates inflammation and fibrosis in a mouse model induced by BLM. (a) Diagram describing the protocol of animal experiment ( mice in each group). (b) H&E staining for observing inflammatory cell infiltration of lung tissues. (c) Masson’s trichrome staining for detecting collagen fibers. (d–f) Serum IL-6 level and IL-1β, and TNF-α in BALF in different groups was measured by ELISA commercial kits. (g, h) The level of FN in lung tissues was measured by Western blot analysis. The bands from three independent experiments were semiquantitatively analyzed by using the ImageJ software, normalized to Tubulin density. Sham: sham surgery with intratracheal administration of normal saline (NS) + oral NS for 7 days; BLM (intratracheal administration of BLM (3 mg/kg) + oral NS); BLM induction and WBT/PFD treatment: BLM + WBT (3, 6, or 12 g/kg)/PFD (pirfenidone 200 mg/kg) for 7 days. (upper image), 100 μm (lower image). , compared to the sham group; , , and compared with the BLM group. All data were analyzed using one-way ANOVA followed by Tukey’s test.

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