Protective Effects of the Wenfei Buqi Tongluo Formula on the Inflammation in Idiopathic Pulmonary Fibrosis through Inhibiting the TLR4/MyD88/NF-κB Pathway

Figure 4

The WBT formula alleviated the NF-κB signaling pathway in BLM-induced mouse model. (a) Protein levels of phosphorylated- NF-κB p65 (p-NF-κB p65), total p65, and IκBα were measured with Western blotting. Tubulin was a loading control. (b, c) The bands of IκBα, p-NF-κB p65, and p65 were semiquantitatively analyzed by using the ImageJ software, normalized to Tubulin density, and calculated relative IκBα expression and the ratio of p-NF-κB p65 and p65. (d) The p-NF-κB p65 expression in lung tissues from different groups was determined by IHC staining. Scale bar: 200 μm (upper image), 100 μm (lower image). BLM: bleomycin. Sham: sham surgery with intratracheal administration of normal saline (NS) + oral NS for 7 days; BLM (intratracheal administration of BLM (3 mg/kg) + oral NS); BLM induction and WBT/PFD treatment: BLM (3 mg/kg) + WBT (3, 6, or 12 g/kg)/PFD (pirfenidone, 200 mg/kg) for 7 days, . , compared to the sham group; , , and compared with the BLM group.

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