Protective Effects of the Wenfei Buqi Tongluo Formula on the Inflammation in Idiopathic Pulmonary Fibrosis through Inhibiting the TLR4/MyD88/NF-κB Pathway

Figure 5

The WBT formula decreased the protein expression of the TLR4/MyD88 pathway in mouse lung tissues induced by BLM. (a) Protein levels of TLR4 and MyD88 were measured by Western blot analysis. (b, c) Quantification of relative expression of TLR4 or MyD88 was performed by densitometric analysis after the normalization of Tubulin. Tubulin was a loading control. (d, e) The levels of TLR4 and MyD88 were detected by immunohistochemical staining in lung tissues from sham (sham surgery with intratracheal administration of normal saline (NS) + oral NS), BLM (intratracheal administration of BLM (3 mg/kg) + oral NS), and WBT (intratracheal administration of BLM (3 mg/kg) + 3, 6, or 12 g/kg WBT) or PFD (intratracheal administration of BLM (3 mg/kg) + pirfenidone 200 mg/kg) for 7 days, . BLM: bleomycin. Scale bar: 200 μm (upper image), 100 μm (lower image). and compared with the BLM group.

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