Human Endothelial Cell Seeding in Partially Decellularized Kidneys

Figure 3

Endothelial recellularization of endothelial decellularized kidneys. (a, b) Histological images of vascular recellularized kidneys with HMEC-GFP. Arrows show the larger endothelial cells. (c–f) Fluorescent microscopy of rat kidney tissue sections stained for green fluorescent protein (bright green), nuclei stained with DAPI (blue), and Evans blue dye (red). The cytoplasm of seeded human cells is seen bright green, whereas the autologous cells present only blue nucleus dyed by DAPI. In (e, f), the extravasation of Evans blue is seen red. (g, h) TUNEL assay of tissue sections of recellularized kidneys. (a–h) A single representative image is shown from 8 recellularized kidneys.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)