miR-338-3p Plays a Significant Role in Casticin-Induced Suppression of Acute Myeloid Leukemia via Targeting PI3K/Akt Pathway

Figure 3

miR-338-3p directly targets RUNX2 in HL-60 cells. (a) The putative binding sites for miR-338-3p in the RUNX2 3-UTR. WT: wild-type; MUT: mutant sequence. (b) The luciferase activity was detected using the dual luciferase reporter assay, after HL-60 cells were cotransfected with a luciferase plasmid harboring RUNX2 3-UTR (WT or MUT) and miR-338 mimics or miR-NC. (c, d) The endogenous expression of RUNX2 was evaluated at mRNA and protein levels by conducting RT-qPCR and Western blot assay, respectively. indicates ; indicates .

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