miR-338-3p Plays a Significant Role in Casticin-Induced Suppression of Acute Myeloid Leukemia via Targeting PI3K/Akt Pathway

Figure 4

Casticin controls tumor growth in vivo by promoting miR-338-3p expression. HL-60 cells were transfected with miR-338 inhibitor, in the presence or absence of casticin. (a) The expression of miR-338-3p in HL-60 cells undergoing different treatment was assessed by qRT-PCR. Compared to untreated cells, ; ; ns means the difference is not significant. The xenograft tumors formed were excised after 4 weeks. (b) The tumor was massed in each group. ; between two groups; “ns” indicates not significant vs. control group. (c) The width and length of tumor were measured, and tumor volume was determined according to them: . ; between two groups; “ns”: not significant vs. control group. (d) The expression level of proteins in the tumor xenografts was detected by western blot assay. Beta-actin was used for the normalization.

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