SOX8 Knockdown Overcomes Enzalutamide Resistance in Castration-Resistant Prostate Cancer by Inhibiting the Notch Signaling Pathway

Figure 3

Knocking down SOX8 inhibits malignant biological behaviors of prostate cancer (PCa) cells that are resistant to treatment. (a, b) The viability of DU145 and Enza-R cells was measured by a CCK-8 assay after knocking down SOX8. (c, d) Colony-forming efficiency of DU145 and Enza-R cells after 10 days of culture. (e) The expression of E-cadherin, N-cadherin, Vimentin, and Zeb-1 in both DU145 and Enza-R cells was examined by Western blot. Cells were transfected with lentiviruses containing LV-NC or LV-shSOX8. GAPDH served as a loading control. (f, g) A Transwell assay was performed to examine the invasive ability of DU145 and Enza-R cells following SOX8 knockdown (magnification, ×400). (h, i). The migratory capacity of Enza-R cells was evaluated after the cells were wounded with a yellow pipette tip for 0 h, 24 h, and 48 h. (j) Enza-R cells were exposed to increasing concentrations of enzalutamide (0, 1, 5, 25, 50, 100, 200, 300, and 400 μM) for 48 h, and the half maximal inhibitory concentration (IC₅₀) was determined by a CCK8 assay. and . Enza-R: enzalutamide-resistant LNCaP cells.

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