SOX8 Knockdown Overcomes Enzalutamide Resistance in Castration-Resistant Prostate Cancer by Inhibiting the Notch Signaling Pathway

Figure 6

Knocking down SOX8 and treatment with RO04929097 resensitized Enza-R and DU145 cells to enzalutamide, while RO04929097 exerts an antitumor effect on resistant cells. (a–d) Enza-R and DU145 cells were subjected to SOX8 knockdown and/or treated with RO04929097. Cells were exposed to increasing concentrations of enzalutamide (0, 1, 5, 25, 50, 100, 200, 300, and 400 μM) for 48 h, and the half maximal inhibitory concentration (IC₅₀) was determined by a CCK-8 assay. (e, f) Enza-R and DU145 cells were treated with increasing concentrations of PF-3084014 (2, 5, 10, 20, 30, 40, 60, 80, and 100 μM) or DMSO for 48 h. Cell viability was detected by a CCK-8 assay. . Enza-R: enzalutamide-resistant LNCaP cells.

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