Research Article
SOX8 Knockdown Overcomes Enzalutamide Resistance in Castration-Resistant Prostate Cancer by Inhibiting the Notch Signaling Pathway
Table 1
Primary antibodies for Western blotting assay.
| | Primary antibodies | Concentration | Company | Catalog number |
| | SOX8 | 1 : 2,000 | Abcam | ab104245 | | Notch4 | 1 : 1,000 | Santa Cruz | SC-383993 | | Hes5 | 1 : 2,000 | Abcam | ab25374 | | E-Cadherin | 1 : 2,000 | CST | 14472 | | N-Cadherin | 1 : 2,000 | CST | 13116 s | | Vimentin | 1 : 1,000 | CST | 5741 s | | Zeb-1 | 1 : 2,000 | CST | 70512 s | | Hey1 | 1 : 2,000 | Abcam | ab22641 | | Hey2 | 1 : 1,000 | Abcam | ab167280 | | P21 | 1 : 2,000 | Abcam | ab109520 | | c-Myc | 1 : 1,000 | Abcam | ab32072 | | Cyclin E1 | 1 : 1,000 | Abcam | ab33911 | | Cyclin D1 | 1 : 2,000 | Abcam | ab16663 | | Cyclin D3 | 1 : 1,000 | Abcam | abSP207 | | BAX | 1 : 2,000 | Abcam | ab53145 | | BAK | 1 : 2,000 | CST | 12105T | | BCL2 | 1 : 2,000 | CST | 4223T | | BCL-xl | 1 : 1,000 | CST | 2764T | | β-Catenin | 1 : 1,000 | Abcam | ab223075 | | p-β-Catenin | 1 : 500 | Abcam | ab277785 | | GAPDH | 1 : 1,000 | CST | 5174s |
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Abcam: Abcam Cambridge, UK; Santa Cruz: Santa Cruz Biotechnology, Inc., USA; CST: Cell Signaling Technology, USA.
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