Bixin Prevents Colorectal Cancer Development through AMPK-Activated Endoplasmic Reticulum Stress

<div>Bixin treatment inhibits the proliferation of CRC cells in vitro. After treatment with indicated doses of Bixin <i>in vitro</i>, HIEC and NCM460 cells (a), as well as CaCO<sub>2</sub> and SW480 cells (b), were used for cell viability determination by the CCK-8 assay. (c) EdU staining was performed in CaCO<sub>2</sub> and SW480 cells after the Bixin treatment. (d) The cell colony formation was analyzed by crystal violet staining. <svg height="8.68572pt" id="M9" style="vertical-align:-0.0498209pt" version="1.1" viewbox="-0.0498162 -8.6359 8.15071 8.68572" width="8.15071pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"></path></g></svg> values: <span class="nowrap"><svg height="11.2224pt" id="M10" style="vertical-align:-1.1199pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 11.2224" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g 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313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,50.025,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> (versus the control group without any treatment).</div>

BioMed Research International

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Figure 1

Figure 1: Bixin Prevents Colorectal Cancer Development through AMPK-Activated Endoplasmic Reticulum Stress 