Bixin Prevents Colorectal Cancer Development through AMPK-Activated Endoplasmic Reticulum Stress

Figure 3

Bixin treatment sensitizes CRC cells to TRAIL-induced apoptosis. CaCO2 and SW480 cells were treated with Bixin at different doses for 24 h, either individually or combined with TRAIL. (a) Cells were stained with Annexin V and PI, and the cell apoptosis was examined by a flow cytometer. (b) Immunoblotting was performed to determine caspase 3 and caspase 9 cleavage levels, as well as Bcl-2 and Bax protein expression levels. values: ; .

(a)

(b)