Bixin Prevents Colorectal Cancer Development through AMPK-Activated Endoplasmic Reticulum Stress

Figure 4

Bixin induces ER stress in CRC cells via AMPK activation. Western blotting analysis was performed to evaluate the expression levels of p-AMPK, p-PERK, CHOP, GRP78, p-eIF2α, and ATF4. (a) CaCO2 and SW480 cells were treated with Bixin at different doses for 24 h, either individually or combined with TRAIL. (b) Bixin and TRAIL combined treatment in CaCO2 and SW480 cells in the absence or presence of 1 μM Compound C (Comp) for 24 h. Each graph represents one of three independently performed experiments.

(a)

(b)