Bixin Prevents Colorectal Cancer Development through AMPK-Activated Endoplasmic Reticulum Stress

Figure 6

Bixin suppresses CRC development in vivo through AMPK activation. Seven days after the xenograft, Bixin (100 mg/kg) was administrated by intraperitoneal (i.p.) injection every 3 days for four times. For AMPK inhibition, Compound C (10 mg/kg) was administered through i.p. injection every day after Bixin administration until sacrifice. 24 d after the xenograft, all mice were euthanized and the tumor (a) volume and (b) weight were measured. (c) p-AMPK, caspase 3, caspase 9, Bcl-2, and Bax expression levels in tumor tissue were determined using immunoblotting. (d) Mice body weight was measured at the end of the treatments. (e) Histological data of H&E staining of the heart, kidney, and liver from different experimental groups. values: ; .

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