Carbon Monoxide-Releasing Molecule-3 Suppresses the Malignant Biological Behavior of Tongue Squamous Cell Carcinoma via Regulating Keap1/Nrf2/HO-1 Signaling Pathway

Figure 2

The effect of CORM-3 on TSCC cell migration and invasion. (a, b) The effect of CORM-3 on the migration of CAL27 and SCC4 cells was tested using scratch assay (magnification, ×40). Statistical analysis of the healing distance showed that CORM-3 treatment could migrate at a lower rate than that in control group and iCORM-3 group. (c, d) The effect of CORM-3 on the invasion of CAL27 cells and SCC4 was tested by transwell assay (magnification, ×200). Cell numbers that invaded the lower membrane surface were less in CORM-3 stimulation groups than in the control group and iCORM-3 group. (e, f) Epithelial mesenchymal transition is involved in the CAL27 and SCC4 cells by CORM-3. CAL27 and SCC4cells were treated with CORM-3 for 0, 0.25, 0.5, 1, 3, 6, and 12 h; the protein expression of E-cadherin and N-cadherin was assessed by western blotting. Relative protein level was normalized to the corresponding GAPDH protein expression. , , and vs. the control group, respectively.

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