[Retracted] Long Intergenic Nonprotein Coding RNA 00174 Aggravates Lung Squamous Cell Carcinoma Progression via MicroRNA-185-5p/Nuclear Factor IX axis

Figure 2

LINC00174 binds to miR-185-5p. (a and b) Subcellular fractionation and FISH assays were utilized to determine the cellular situation of LINC00174. (c) Possible miRNAs combined with LINC00174 were predicted from starBase. (d) RNA pull down assay was performed to study the affinity between LINC00174 and potential combinable miRNAs. (e) RT-qPCR analysis was performed to detect miR-185-5p expression in LUSC cells. (f) Binding sites between LINC00174 and miR-185-5p were displayed. (g) Luciferase reporter assays were performed to verify the interaction between LINC00174 and miR-185-5p. .

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