[Retracted] Long Intergenic Nonprotein Coding RNA 00174 Aggravates Lung Squamous Cell Carcinoma Progression via MicroRNA-185-5p/Nuclear Factor IX axis

Figure 3

NFIX is the target gene of miR-185-5p. (a) RNA22, micro T, and Target Scan tools predicted 8 potential targets of miR-185-5p. (b) RT-qPCR detected the expression of mRNA candidates after overexpressing miR-185-5p. (c) RT-qPCR evaluated NFIX expression in LUSC cells. (d) RIP assay detected the enrichment of LINC00174, miR-185-5p, and NFIX in anti-IgG and anti-Ago 2 groups. (e) Binding sites between miR-185-5p and NFIX 3UTR were displayed. (f) Dual luciferase reporter assays determined the relationship between miR-185-5p and NFIX 3UTR. (g) RT-qPCR analysis was performed to study the effects of sh-NC, sh-LINC00174#2, and sh-LINC00174#2+miR-185-5p inhibitor on NFIX expression. .

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