[Retracted] Long Intergenic Nonprotein Coding RNA 00174 Aggravates Lung Squamous Cell Carcinoma Progression via MicroRNA-185-5p/Nuclear Factor IX axis

Figure 4

LINC00174 aggravates LUSC progression via regulating NFIX. (a) RT-qPCR examined NFIX expression in LUSC cells after transfecting with pc DNA3.1 and pcDNA3.1/NFIX. Rescue assays were performed with LUSC cells after transfection of sh-NC, sh-LINC00174#2, and sh-LINC00174#2+pcDNA3.1/NFIX, respectively. (b and c) RT-qPCR and western blot determined the expression of NFIX. (d and e) Colony formation and EdU experiments detected the cell proliferation. (f) TUNEL assay detected the apoptosis. (g and h) Transwell assays detected the cell migration and invasion. (i) Xenograft tumor growth curve was shown. (j and k) Xenograft tumor volume and weight were measured. .

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