Evaluation of Genotoxic Effect and Antigenotoxic Potential against DNA Damage of the Aqueous and Ethanolic Leaf Extracts of Annona muricata Using an In Vivo Erythrocyte Rodent Micronucleus Assay

Figure 2

Experimental design to evaluate in vivo the genotoxicity, cytotoxicity, and antimutagenicity of A. muricata aqueous and ethanolic leaf extracts in mice peripheral blood. Genotoxic and cytotoxic evaluation. Groups: negative control sterile water for five days; 2-7: one of the following doses (187.5, 375, and 750 mg/kg) of A. muricata aqueous and ethanolic leaf extracts for 5 days; PC: positive control 60 mg/kg of CP divided into two doses of 30 mg/kg, once daily for two days. Antigenotoxic evaluation. Groups: mice given 1 h prior to CP administration (60 mg/kg of CP divided into two doses of 30 mg/kg) once daily for two days one of the following doses (187.5, 375, and 750 mg/kg) of aqueous and ethanolic leaf extracts of A. muricata once daily for 5 days.