Evaluation of Genotoxic Effect and Antigenotoxic Potential against DNA Damage of the Aqueous and Ethanolic Leaf Extracts of Annona muricata Using an In Vivo Erythrocyte Rodent Micronucleus Assay

Table 4

Evaluation of the antigenotoxic effect and %DR of A. muricata aqueous and ethanolic leaf extracts against CP-induced damage in MNPCEs cell count in mouse peripheral blood.

MNPCEs/1000 PCEs
Sampling time
	Treatments	0 h	24 h	48 h	72 h	96 h	120 h
NC; : 5	Sterile water
			NS	NS	NS	NS	NS
PC; : 5	CP
	60 mg/kg		NS	NS	NS	NS	0.043
CP+aqueous extract A. muricata; : 5	60 mg/kg+187.5 mg/kg
			NS	0.001	0.001	NS	NS
	%DR		34.48%	61.22%	67.50%	48.84%	16.67%
	60 mg/kg+375 mg/kg
			NS	0.031	0.001	0.037	NS
	%DR		48.28%	42.86%	62.50%	55.81%	61.11%
	60 mg/kg+750 mg/kg
			NS	NS	NS	0.002	0.037
	%DR		37.93%	28.57%	51.25%	55.81%	11.11%
CP+EtOH extract A. muricata; : 5	60 mg/kg+187.5 mg/kg
			NS	NS	0.001	0.001	0.004
	%DR		34.48%	68.75%	68.75%	72.09%	61.11%
	60 mg/kg+375 mg/kg
		NS	NS	0.015	0.001	0.011	NS
	%DR		41.38%	46.94%	67.50%	65.12%	72.22%
	60 mg/kg+750 mg/kg
			NS	NS	0.011	0.001	0.008
	%DR		24.14%	48.98%	70.00%	67.44%	33.33%

Data are expressed as . One-way ANOVA, followed by the Dunnett -test for multiple post hoc pairwise comparisons versus the PC, was employed to correct the significance () values for intergroup analysis. %DR: percentage of damage reduction; CP: cyclophosphamide; MNPCEs: micronucleated polychromatic erythrocytes; n: mice per group; NC: negative control; NS: not significant; PC: positive control; PCEs: polychromatic erythrocytes.