Evaluation of Genotoxic Effect and Antigenotoxic Potential against DNA Damage of the Aqueous and Ethanolic Leaf Extracts of Annona muricata Using an In Vivo Erythrocyte Rodent Micronucleus Assay

Table 5

Evaluation of the antigenotoxic effect and %DR of A. muricata aqueous and ethanolic leaf extracts against CP-induced damage in MNEs cell count in mouse peripheral blood.

MNEs/10,000 TEs
Sampling time
	Doses	0 h	24 h	48 h	72 h	96 h	120 h
NC; : 5	Sterile water
PC; : 5	CP
CP+aqueous extract A. muricata; : 5	60 mg/kg+187.5 mg/kg
			NS	NS	0.010	0.001	0.001
	%DR		62.26%	64.56%	54.82%	84.21%	46.38%
	60 mg/kg+375 mg/kg
			NS	NS	0.001	0.001	0.001
	%DR		52.83%	81.01%	86.80%	81.95%	62.32%
	60 mg/kg+750 mg/kg
			NS	NS	0.010	0.001	0.002
	%DR		47.17%	59.49%	68.02%	69.17%	40.58%
CP+EtOH extract A. muricata; : 5	60 mg/kg+187.5 mg/kg
			NS	NS	NS	0.001	0.003
	%DR		35.85%	44.30%	55.84%	65.41%	60.87%
	60 mg/kg+375 mg/kg
		NS	NS	0.002	0.001	0.009	NS
	%DR		66.04%	70.89%	65.48%	57.89%	62.32%
	60 mg/kg+750 mg/kg
			0.037	NS	0.059	0.001	NS
	%DR		58.49%	46.84%	57.36%	43.61%	31.88%

Data are expressed as . One-way ANOVA followed by the Dunnett -test for multiple post hoc pairwise comparisons versus the PC was employed to correct the significance () values for intergroup analysis. %DR: percentage of damage reduction; CP: cyclophosphamide; MNEs: micronucleated erythrocytes; : mice per group; NC: negative control; NS: not significant; PC: positive control; TEs: total erythrocytes.