Evaluation of Genotoxic Effect and Antigenotoxic Potential against DNA Damage of the Aqueous and Ethanolic Leaf Extracts of Annona muricata Using an In Vivo Erythrocyte Rodent Micronucleus Assay

Table 6

Frequencies of MNPCEs/1,000 PCEs in pregnant rats peripheral blood study groups treated with different doses of A. muricata aqueous and ethanolic leaf extracts at the different sampling times.

MNPCEs/1000 PCEs
Sampling time
	Treatments	0 h	24 h	48 h	72 h	96 h	120 h
NC; : 5	Sterile water
			NS	NS	NS	NS	NS
Aqueous extract A. muricata; : 5	187.5 mg/kg
			NS	NS	NS	NS	NS
	375 mg/kg
			NS	NS	NS	NS	NS
	750 mg/kg
			NS	NS	NS	NS	NS
EtOH extract A. muricata; : 5	187.5 mg/kg
			NS	NS	NS	NS	NS
	375 mg/kg
			NS	NS	NS	NS	NS
	750 mg/kg
			NS	NS	NS	NS	NS
PC; : 5	CP
	60 mg/kg		NS	NS	NS	0.001	0.001

Data are expressed as . Intragroup comparisons were performed between baseline samples (0 h) against the following sampling times: 24, 48, 72, 96, and 120 h (ANOVA Bonferroni test post hoc for multiple comparisons, ). A. muricata: Annona muricata; CP: cyclophosphamide; MNPCEs: micronucleated polychromatic erythrocytes; : mice per group; NC: negative control; NS: not significant; PCEs: polychromatic erythrocytes; PC: positive control.