Integrated Analysis of Multiple Microarray Studies to Identify Core Gene-Expression Signatures Involved in Tubulointerstitial Injury in Diabetic Nephropathy

Figure 6

GSEA analysis and identified core gene related to the renal tubulointerstitial injury. GSEA plot showing most enriched gene sets from c2.cp.kegg.v7.4.symbols.gmt in the DN group. (a) The top 8 significant enriched gene set positively correlated with the DN group was KEGG_PATHOGENIC_ESCHERICHIA_COLI_INFECTION (, , , ), KEGG_FC_GAMMA_R_MEDIATED_PHAGOCY-TOSIS (, , , ), KEGG_FOCAL_ADHESION (, , , ), KEGG_FC_EPSILON_RI_ SIGNALING_PATHWAY (, , , ), KEGG_CYTOSOLIC_DNA_SENSING_PATHWAY (, , , ), KEGG_CELL_CYCLE (, , , ) (, , , ) (, , , ), KEGG_VIRAL_MYOCARDITIS (, , , ), and KEGG_NATURAL_KILLER_CELL_MEDIATED_ CYTOTOXICITY (, , , ). (b) Venn diagram of genes in WGCNA-focal adhesion pathway, GSEA-focal adhesion pathway, and the related genes of the top 30 nodes with neighbors and expanded ranked by degree method in “cyto-hubba” of PPI network. (c) Venn diagram of genes in WGCNA-viral myocarditis pathway, GSEA-viral myocarditis pathway, and the related genes of the top 30 nodes with neighbors and expanded ranked by degree method in “cyto-hubba” of PPI network. (d) The heat map of final 17 core genes in GSE30529-47184. WGCNA-focal adhesion: genes in focal adhesion pathway by WGCNA analysis; GSEA-focal adhesion: genes in focal adhesion pathway by GSEA KEGG analysis; WGCNA-viral myocarditis: genes in viral myocarditis pathway by WGCNA analysis; GSEA-viral myocarditis: genes in viral myocarditis pathway by GSEA KEGG analysis; PPI-degree top 30: the related genes of the top 30 nodes with neighbors and expanded ranked by degree method in “cyto-hubba” of PPI network; red: upregulation; blue: downregulation.

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