Differential Expression and Bioinformatics Analysis of tRF/tiRNA in Endometriosis Patients
Table 2
Under the conditions of , compared with the control group, there were a total of 18 tRF/tiRNA differential expressions in the experimental group samples, of which 2 tRF/tiRNAs were upregulated and 16 were downregulated.
AVG ()
Fold difference
-test
Fold up- or Downregulation
Transcript name
Well
Test
Control
Test
Control
Test/control
value
Test/control
Comments
3tiR_012_ArgCCT (n)
A02|B02
12.90
11.88
0.0001305622
0.0002651040
0.49
0.0217
-2.03
OKAY
3tiR_026_GlnCTG (n)
A03|B03
11.50
10.72
0.0003449815
0.0005910585
0.58
0.0033
-1.71
OKAY
3tiR_056_ValTAC (mt)
A06|B06
7.65
6.25
0.0049731201
0.0131295495
0.38
0.0281
-2.64
OKAY
3tiR_078_ArgTCT (n)
A10|B10
7.65
7.00
0.0049754532
0.0078167154
0.64
0.0384
-1.57
OKAY
5008C
A15|B15
10.77
8.90
0.0005734410
0.0020932464
0.27
0.0445
-3.65
OKAY
5016C
A17|B17
16.50
15.16
0.0000108028
0.0000273061
0.40
0.0389
-2.53
B
TRF419
A23|B23
13.85
12.21
0.0000676452
0.0002109906
0.32
0.0439
-3.12
B
tiRNA-5033-ProTGG-1
A24|B24
7.94
7.04
0.0040641490
0.0076003464
0.53
0.0018
-1.87
OKAY
tiRNA-5031-PheGAA
C17|D17
10.47
9.68
0.0007030109
0.0012169640
0.58
0.0382
-1.73
OKAY
tiRNA-5030-GlnTTG-3
C19|D19
16.60
18.34
0.0000100786
0.0000030114
3.35
0.0403
3.35
OKAY
1001
E08|F08
19.05
17.23
0.0000018452
0.0000065217
0.28
0.0478
-3.53
B
1030
E24|F24
18.85
16.97
0.0000021147
0.0000078082
0.27
0.0009
-3.69
B
1035
G04|H04
19.82
18.15
0.0000010836
0.0000034430
0.31
0.0204
-3.18
C
1042
G11|H11
19.21
16.81
0.0000016479
0.0000087138
0.19
0.0016
-5.29
B
3033A
G16|H16
10.14
9.42
0.0008843119
0.0014609092
0.61
0.0407
-1.65
OKAY
3006A
G22|H22
11.76
10.52
0.0002879253
0.0006802099
0.42
0.0109
-2.36
OKAY
3008B
G24|H24
19.30
17.56
0.0000015512
0.0000051712
0.30
0.0362
-3.33
OKAY
3011/12A
I02|J02
6.64
6.21
0.0100170904
0.0135292833
0.74
0.0050
-1.35
OKAY
TRF308
M06|N06
17.47
15.57
0.0000055225
0.0000206195
0.27
0.0306
-3.73
C
TRF320
M10|N10
18.89
16.82
0.0000020591
0.0000086166
0.24
0.0177
-4.18
C
TRF396
O02|P02
14.01
16.51
0.0000607708
0.0000107517
5.65
0.0070
5.65
C
“OKAY”: the average Ct is relatively high (>30) in one of the samples but is still relatively low in the other sample (<30), suggesting that the fold change value may not be as accurately calculated. “B”: the average Ct values are relatively high (>30) in both control and test samples, and the value is either unavailable or above cut-off (). More biological replicates are needed to detect differential expression at low expression levels. “C”: the average Ct values are not determined or greater than the cut-off (default 35) in both samples, indicating that the gene expression is undetected.
