SMAD1 Loss-of-Function Variant Responsible for Congenital Heart Disease

Table 3

Primers for amplification of the coding exons as well as splicing junctions of the SMAD1 gene.

Coding exons	Forward primers (5 → 3)	Backward primers (5 → 3)	Amplicons (bp)
1	TGTCCTTTTGCATTTGGAGAC	CAAATCTGGTACTGGGCACAC	518
2	TTGAGTTGGCAGCAGGACAG	ACTGCAGGTTGACCCAGCTT	473
3	GGCAGTGCCTGTAGCCTTTAG	CCAGCAATTGTAGTGAGCTTCT	412
4	CCATGATCCTGAGCCAATTC	C	483
5	CTGTGTTGAAGCTGCACAGG	TGCAAGAGCTTCCAGATAGCAG	590
6	CAGGGAGGAAAGATGCATAG	AGCGGTGCTATCTGAATAAGGA	569
	C	ACAATTTGTCCCTGGCTTGG