The Ecto-5nucleotidase/CD73 Mediates Leishmania amazonensis Survival in Macrophages

Figure 1

Expression of CD39 and CD73 in naïve macrophages in in vivo. Resident macrophages were harvested from the peritoneum of naïve C57BL/6 mice using ice-cold 10 ml PBS. After centrifugation and resuspension in 0.2% bovine serum albumin (0.2% BSA/PBS) and Fc-blocked (purified rat anti-mouse CD16/CD32, clone 2.4G2, BD Pharmingen), the harvested cells were labeled with anti-murine F4/80, anti-CD39, and anti-CD73 antibodies and then were analyzed by flow cytometry. Indicated in the figure, the percentage of cells expressing (a) CD39, (b) CD73, and (c) CD39CD73 in F4/80⁺ and F4/80^- cells from total peritoneal population. This result is the of at least 3 independent experiments. means the statistical difference in the expression of CD39 and CD73 between F4/80⁺ and F4/80^- cell populations using paired two-tailed Student’s -test.

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